gene exp fgfr4 hs01106908 m1  (Thermo Fisher)

Journal: Communications Biology

Article Title: Wnt/FGF20 signaling axis promotes migration of proximal blastema cell during tail regeneration of Gekko japonicus

doi: 10.1038/s42003-025-09360-6

Figure Lengend Snippet: a qRT-PCR results showed that SKL2001 treatment reduced fgfr4 mRNA levels by 31.4% in cultured blastema cells. Data were analyzed using an unpaired Student’s t-test and are presented as mean ± SEM, n = 5. The P value is shown on the panel . b qRT-PCR analysis of fgfr4 mRNA levels in the 1 mm tip portion of the distal blastema on days 8, 10, 12, 14 post-autotomy. Data were analyzed using one-way ANOVA followed by Dunnett’s post hoc test and are presented as mean ± SEM, n = 5. The P value is shown on the panel. c Representative immunofluorescence and EdU staining images showed FGFR4 (green) expression and proliferating cells (EdU, violet) in regenerated tissues on days 8, 10, 12, and 14 post-autotomy. The region between the solid and dotted lines represent WE. Scale bars represent 50 µm. d Normalized FGFR4 fluorescence intensity of z1-z5 on day 14. Integrated density was divided by the corresponding cell area and expressed as fluorescence intensity per unit area (arbitrary units, a. u.). The mean value of the control group was set to 1. Data were analyzed using one-way ANOVA followed by Dunnett’s post hoc test and are presented as mean ± SEM, n = 5. The P value is shown on the panel. e Schematic timeline for DMSO/IWP4 injection and sample collection. f qRT-PCR results showed a 67.2% increase in the mRNA levels of fgfr4 in the blastema after Wnt/β-catenin inhibition. Data were analyzed using an unpaired Student’s t -test and are presented as mean ± SEM, n = 5. The P value is shown on the panel. WE, wound epithelium. NC, negative control. qRT-PCR, quantitative reverse transcription.

Article Snippet: The Wnt inhibitor IWP-4 (2 mg/kg body weight, Cat# E0033, Selleck Chemicals, Houston, TX) or FGFR4 specific inhibitor BLU9931 (100 mg/ kg body weight, Cat# HY-12823, MedChemExpress, Monmouth Junction, NJ, USA) dissolved in 5% dimethylsulphoxide (DMSO) saline solution was injected intraperitoneally every other day from day 3 post-autotomy.

Techniques: Quantitative RT-PCR, Cell Culture, Immunofluorescence, Staining, Expressing, Fluorescence, Control, Injection, Inhibition, Negative Control, Reverse Transcription

Journal: Communications Biology

Article Title: Wnt/FGF20 signaling axis promotes migration of proximal blastema cell during tail regeneration of Gekko japonicus

doi: 10.1038/s42003-025-09360-6

Figure Lengend Snippet: a Schematic timeline for DMSO/BLU9931 injection and sample collection. b The regeneration stage were observed on days 8 and 12 post-autotomy. The results of statistical analysis showed that FGFR4 inhibitor BLU9931 delayed tail regeneration. Data were analyzed by χ 2 test. The P values are shown on the panel. c EdU staining (red) of proliferating cells in DMSO group and BLU9931-treatment group. d Quantification of EdU-positive cells in WE and blastema. In DMSO group, the percentage of EdU-positive cells in WE = (56.9 ± 3.5) %, blastema = (48.3 ± 4.2) %. In BLU9931 group, the percentage of EdU-positive cells in WE = (24.7 ± 2.5) %, blastema = (25.9 ± 3.2) %. The results indicated that BLU9931 treatment suppressed cell proliferation in both wound epithelium and the blastema. Data were analyzed using two-way ANOVA followed by Bonferroni’s post hoc test and are presented as mean ± SEM, n = 5. The P values are shown on the panel. e , f EdU assay of proliferating cells after treatment with Wnt agonist SKL2001 combined with fgfr4 over-expression (OE- fgfr4 ) in vitro and quantification of EdU-positive cells showed that fgfr4 over-expression alleviated the inhibitory effect of Wnt signaling on cell proliferation. Data were analyzed using two-way ANOVA followed by Bonferroni’s post hoc test and are presented as mean ± SEM, n = 5, The P values are shown on the panel. WE, wound epithelium.

Article Snippet: The Wnt inhibitor IWP-4 (2 mg/kg body weight, Cat# E0033, Selleck Chemicals, Houston, TX) or FGFR4 specific inhibitor BLU9931 (100 mg/ kg body weight, Cat# HY-12823, MedChemExpress, Monmouth Junction, NJ, USA) dissolved in 5% dimethylsulphoxide (DMSO) saline solution was injected intraperitoneally every other day from day 3 post-autotomy.

Techniques: Injection, Staining, EdU Assay, Over Expression, In Vitro

Journal: Communications Biology

Article Title: Wnt/FGF20 signaling axis promotes migration of proximal blastema cell during tail regeneration of Gekko japonicus

doi: 10.1038/s42003-025-09360-6

Figure Lengend Snippet: Wnt signaling is activated in the distal blastema cells, leading to a reduction in the proliferation of distal blastema cells adjacent to the wound epithelium through the downregulation of FGFR4. Additionally, FGF20, induced by Wnt signaling, promotes the migration of proximal blastema cells.

Article Snippet: The Wnt inhibitor IWP-4 (2 mg/kg body weight, Cat# E0033, Selleck Chemicals, Houston, TX) or FGFR4 specific inhibitor BLU9931 (100 mg/ kg body weight, Cat# HY-12823, MedChemExpress, Monmouth Junction, NJ, USA) dissolved in 5% dimethylsulphoxide (DMSO) saline solution was injected intraperitoneally every other day from day 3 post-autotomy.

Techniques: Migration